Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. I'm Dr Ashley Mayer . I'm a practicing integrative physician, and also a mama to two great kiddos of my own! If you caught my post on Ibuprofen a few days ago, The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link