Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Acetaminophen Induced Hepatotoxicity: a Comprehensive Update Your livers unsung hero: Glutathione , Lets talk about what really happens when you take Tylenol (acetaminophen)., Your liver breaks down acetaminophen through several pathways. One pathway creates